Single Plane Illumination Microscope

Single Plane Illumination Microscopes (SPIM) uses a "sheet" of light to illuminate a single plane of a 3D sample while the detection of fluorescence is performed prependicular. SPIM provides fast and 'true' optical sectioning of large samples with minimal photobleaching and phototoxicity effect. Ideal for large and cleared specimens like developing systems, organoids and spheroids. Imaging of live samples is aslo available.

• LaVision UMII mounted with a 16x water immersed objective and 1x or 2x zoom body
• Andor Neo 5.5 sCMOS camera
• Dual-side illumination with 6 independent light-sheets for reducing shadow and stripe artifacts
• Adjustable light-sheet width
• Adjustable light-sheet waist thickness (~ 4-15 um)
• Horizontal focus for uniform axial resolution accross the whole field of view
• 3 laser lines (488nm, 561nm, 642nm)
• Mosaic acquisition and stiching for imaging big samples
• Incubation chamber for in vivo imaging with controlled conditions of O2, CO2 and temperature
• In-house-build module for multi-view acquisition
• Acquisition software: ImSpectorPro