X-Light V3/DeepSIM

The DeepSIM super resolution module is integrated with the X-Light V3 Spinnign Disk Confocal system, providing enchanched imaging of sub-cellular structures. It also allows the examination of live-cell dynamics, with an improved resolution up to 100nm in XY, without requiring any special sample preparation or fluorescent dyes.

• Nikon ECLIPSE Ti2 inverted microscope mounted with 4x, 10x, 20x, 40x, 60x, 100x objective lenses
• DualCam module using two Kinetix cameras, offering Hight Sensitivity, large FOV and fast acquisition speeds.
• DeepSIM X-Light module, with 3-different difraction patterns, by CrestOptics
• X-Light V3 Spinnign Disk Confocal unit, by CrestOptics
• Module for photobleaching (FRAP) and photoactivation experiments (PA)
• 6 laser lines (405nm, 488nm, 561nm, 638nm)
• Enviromental chamber to control CO2 levels and temperature
• Acquisition software: NIS-Elements V2.3

The DeepSIM microscope is funded by:

• "Translation Discovery hub: Open-Access research Infrastructure for translational research for the discovery and development of targeted therapeutic molecules"
• Project: "Democritus University of Thrace - University of Excellence" / SUB2: UNIVERSITIES OF EXCELLENCE - UoEs
• Funding: 1,000,000 Euros
• Scientific co-ordinator: Professor Michalis Koukourakis (Department of Medicine, DUTH)

The use of the DeepSIM microscope is subject to the General Open Access Regulation of the Democritus University of Thrace.