X-Light V3/DeepSIM

The DeepSIM super resolution module is integrated with the X-Light V3 Spinnign Disk Confocal system, providing enchanched imaging of sub-cellular structures. It also allows the examination of live-cell dynamics, with an improved resolution up to 100nm in XY, without requiring any special sample preparation or fluorescent dyes.

• Nikon Eclipse Ti2 Fully Automated inverted microscope mounted with 10x, 20x, 40x, 60x Silicon and 60x Oil objective lenses.
• Crest X-light V3 spinning disk confocal system, suitable for simultaneous use of two cameras.
• Two (2) Photometrics Kinetix cameras, offering Hight Sensitivity, large FOV and fast acquisition speeds.
• Omicron BriXXHub laser light source with emission wavelengths at 405/445/488/555/640/735 nm
• DeepSIM Super-Resolution system by CrestOptics, with 3-different difraction patterns, and with software for 3D deconvolution.
• OMS LAPP-VIS system for FRAP and Photostimulation with all required filters.
• Piezoelectric focusing system (Nano-Z) with a 200 µm range by Mad City Labs.
• High-end computer HP Z4 G5 with HP S7 Pro 738pu WQHD+ monitor.
• Enclosure box for temperature and CO₂ control from EMBL.
• Vibration isolation table.
• Acquisition software: NIS-Elements V2.3.
• For the detailed system configuration click here

The DeepSIM microscope is funded by:

• "Translation Discovery hub: Open-Access research Infrastructure for translational research for the discovery and development of targeted therapeutic molecules"
• Project: "Democritus University of Thrace - University of Excellence" / SUB2: UNIVERSITIES OF EXCELLENCE - UoEs
• Funding: 1,000,000 Euros
• Scientific co-ordinator: Professor Michalis Koukourakis (Department of Medicine, DUTH)

The use of the DeepSIM microscope is subject to the General Open Access Regulation of the Democritus University of Thrace.