Stochastic Optical Reconstruction Microscope

Stochastic Optical Reconstruction Microscopy (STORM) is a super-resolution fluorescence imaging technique that overcomes the diffraction limit of light by localizing individual fluorophores with nanometer precision, enabling visualization of cellular structures at the molecular scale.

• Nikon ECLIPSE Ti2 inverted microscope mounted with 4x, 10x, 20x, 40x, 60x, 100x objective lenses
• TwinCam module with Andor Ixon-897 EM-CCD and TBD
• Total Internal Reflection Fluorescence (TIRF) imaging is available
• 4 laser lines (405nm, 488nm, 561nm, 638nm)
• Acquisition software: NIS-Elements V2.3

The DeepSIM microscope is funded by:

• "Translation Discovery hub: Open-Access research Infrastructure for translational research for the discovery and development of targeted therapeutic molecules"
• Project: "Democritus University of Thrace - University of Excellence" / SUB2: UNIVERSITIES OF EXCELLENCE - UoEs
• Funding: 1,000,000 Euros
• Scientific co-ordinator: Professor Michalis Koukourakis (Department of Medicine, DUTH)

The use of the STORM microscope is subject to the General Open Access Regulation of the Democritus University of Thrace.